Investigating the Correlation between Post-mortem Interval and RIN Values: A Pilot Study

In this human post-mortem autopsy study, 10 organ tissues from 4 cases with different Post-Mortem Intervals (PMIs) were evaluated. Clinical history, agonal state, refrigeration time, and PMI of 10, 12, 16, >24 hours evaluated to determine histology molecular markers the tissue. Results indicated was not predictive RNA stability (RIN values). We observed time after death influenced body temperature, delayed autolysis, increased Integrity Number (RIN) values. However, prolonged intervals hypoxemia decreased RIN medical history state scores two most important variables affecting The 16 > 24-hour PMIs for all had greater than 80% autolysis in pancreas small intestine compared 10- 12-hour minimal moderate (25-40%). Of evaluated, highest average values heart (7.1), lung (5.9), skin (5.9). changes during collection positively affected integrity histological samples. Therefore, it is necessary minimize sample at room maximize refrigeration, intervals. tissues, except GI tract, highly correlated a but greatly by case study. Prompt may assist preserving both